A new multiplex PCR for species-specific diagnosis of human candidiasis
نویسندگان
چکیده
منابع مشابه
Comparison of Two Multiplex PCR Systems for Meat Species Authentication
Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication. Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...
متن کاملA real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...
متن کاملGroup-specific Multiplex Pcr Identification of Canine Faecal Lactobacillus Species
A set of forty-nine Lactobacillus isolates recovered from the faecal swabs of different dog breeds viz. Pomeranian (12), Labrador (15) and mongrel/indigenous dogs (22) were subjected to Lactobacillus group-specific multiplex PCR corresponding to the 16S-23S rRNA intergenic spacer region (ISR) and its flanking 23S rRNA gene. Twenty-three (46.9%) isolates were found to be positive for Group IV; s...
متن کاملIdentification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
BACKGROUND Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. METHODS 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fra...
متن کاملConventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat
Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...
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ژورنال
عنوان ژورنال: Biomédica
سال: 2017
ISSN: 0120-4157,0120-4157
DOI: 10.7705/biomedica.v37i2.3202